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KMID : 0613820030130060843
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Journal of Life Science
2003 Volume.13 No. 6 p.843 ~ p.848
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Isolation, Identification and Cultural Condition of the Antagonistic Microorganism Against Salmonella gallinarum Causing Fowl Typhoid
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Kim Jin-Rack
Kim Sang-Dal
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Abstract
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Diarrhea and death of chicken have been brought about by fowl typhoid caused by Salmonella gallinarum, which causes a great loss of chicken farms. For the development of the probiotic which can control a fowl typhoid of S. gallinarum without any adverse effect of commercial existing antibiotics, we isolated antagonistic intestinal bacteria against S. gallinarum from a bowel of the chicken which was pastured in a chicken farm of Gumi, Kyoungbuk. An Y3 strain which had a strong antagonistic ability to S. gallinarum was selected as a candidate of chicken probiotic microorganism among isolated strains. It was identified as a Bacillus amyloliquefaciens by 98% similarity by the result of cultural, physiological, biochemical test and Biolog system(MicrologTM 4.0), and named as Bacillus amyloliquefaciens Y3. The strain showed the strongest antagonistic activity and a good growth at pH 5-9, 37℃.
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KEYWORD
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fowl typhoid, antagonistic bacteria, Salmonella gallinarum
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